Abstract
Rapid, site-selective modification of cysteine residues with chloromethyl-triazole derivatives generates pseudo-acyl sLys motifs, mimicking important post-translational modifications. Near-native biotinylation of peptide and protein substrates is shown to be site-selective and modified histone H4 retains functional activity.
MeSH terms
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Acylation
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Biotinylation
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Cysteine / chemistry*
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Histones / chemistry*
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Humans
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Peptide Fragments / chemistry
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Peptide Fragments / metabolism*
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Protein Processing, Post-Translational*
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Proteins / chemistry
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Proteins / metabolism*
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Triazoles / chemistry*
Substances
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Histones
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Peptide Fragments
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Proteins
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Triazoles
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Cysteine