Aluminum trichloride induces bone impairment through TGF-β1/Smad signaling pathway

Abstract

Aluminum (Al) is recognized worldwide as serious inorganic contaminants. Exposure to Al is associated with low BMD and an increased risk of osteoporosis. However, the precise molecular mechanisms remains unclear. Thus, in this study, rats were orally exposed to 0 (control group, CG) and 0.4g/L AlCl₃ (AlCl₃ treated group, AG) in drinking water for 120days; osteoblasts were treated with AlCl₃ (0.12mg/mL) and/or TGF-β1 (4.5ng/mL) for 24h. We found that AlCl₃ decreased the BMD, damaged femoral ultrastructure, decreased the activities of GSH-Px and SOD, and increased the levels of ROS and MDA in bone, decreased the activity of B-ALP and content of PINP, and increased the activity of TRACP-5b and content of NTX-I in serum, decreased mRNA expressions of TGF-β1, TβRI, TβRII and Smad4, protein expressions of TGF-β1, p-Smad2/3 and Smad2/3/4 complex, and increased Smad7 mRNA expression in bone and in osteoblasts. Moreover, we found exogenous TGF-β1 application reversed the inhibitory effect of AlCl₃ on osteoblasts activity by activating the TGF-β1/Smad signaling pathway and increasing the mRNA expressions of ALP and Col I in osteoblasts. These results demonstrate that AlCl₃ induces bone impairment through inactivation of TGF-β1/Smad signaling pathway.

Keywords: Aluminum trichloride; Bone formation and resorption; Osteoblasts; Oxidative stress; TGF-β1/Smad signaling pathway.