A robust and straightforward approach for fabrication of a new type of colloidal pressure sensor was proposed. For this purpose, we synthesized uniform conductive magnetic-patchy microparticles using a microfluidic technique and then coated them with poly(3,4-ethylene dioxythiophene)-poly(styrene sulfonate) layers using the layer-by-layer deposition. Finally we showed that the magnetic-patchy conductive microparticles could be positioned on the target sites while precisely detecting pressure changes with excellent sensitivity.