Enhancement of pectinase production by ultraviolet irradiation and diethyl sulfate mutagenesis of a Fusarium oxysporum isolate

Genet Mol Res. 2016 Sep 23;15(3). doi: 10.4238/gmr.15038986.

Abstract

Fusarium oxysporum strain BM-201 was treated with ultraviolet (UV) radiation to obtain a high pectinase-producing strain. Mutant UV-10-41 was obtained and then treated by diethyl sulfate. Next, the mutant UV-diethyl sulfate-43 derived from UV-10-41 was selected as high pectinase-producing strain. Mutant UV-diethyl sulfate-43 was incubated on slant for 10 generations, demonstrating that the pectinase-producing genes were stable. Pectinase activity reached 391.2 U/mL, which is 73.6% higher than that of the original strain.

MeSH terms

  • Dose-Response Relationship, Radiation
  • Fermentation / drug effects
  • Fermentation / radiation effects
  • Fusarium / enzymology*
  • Fusarium / genetics*
  • Fusarium / isolation & purification
  • Fusarium / radiation effects
  • Hexuronic Acids / metabolism
  • Mutagenesis / drug effects
  • Mutagenesis / genetics*
  • Mutagenesis / radiation effects
  • Polygalacturonase / biosynthesis*
  • Reference Standards
  • Sulfuric Acid Esters / toxicity*
  • Time Factors
  • Ultraviolet Rays*

Substances

  • Hexuronic Acids
  • Sulfuric Acid Esters
  • galacturonic acid
  • Polygalacturonase
  • diethyl sulfate