Abstract
Fusarium oxysporum strain BM-201 was treated with ultraviolet (UV) radiation to obtain a high pectinase-producing strain. Mutant UV-10-41 was obtained and then treated by diethyl sulfate. Next, the mutant UV-diethyl sulfate-43 derived from UV-10-41 was selected as high pectinase-producing strain. Mutant UV-diethyl sulfate-43 was incubated on slant for 10 generations, demonstrating that the pectinase-producing genes were stable. Pectinase activity reached 391.2 U/mL, which is 73.6% higher than that of the original strain.
MeSH terms
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Dose-Response Relationship, Radiation
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Fermentation / drug effects
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Fermentation / radiation effects
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Fusarium / enzymology*
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Fusarium / genetics*
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Fusarium / isolation & purification
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Fusarium / radiation effects
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Hexuronic Acids / metabolism
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Mutagenesis / drug effects
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Mutagenesis / genetics*
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Mutagenesis / radiation effects
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Polygalacturonase / biosynthesis*
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Reference Standards
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Sulfuric Acid Esters / toxicity*
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Time Factors
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Ultraviolet Rays*
Substances
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Hexuronic Acids
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Sulfuric Acid Esters
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galacturonic acid
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Polygalacturonase
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diethyl sulfate