Complete genome sequence of a KI polyomavirus isolated from an otherwise healthy child with severe lower respiratory tract infection

J Med Virol. 2017 May;89(5):926-930. doi: 10.1002/jmv.24706. Epub 2016 Nov 9.

Abstract

Unbiased, deep sequencing of a nasal specimen from an otherwise healthy 13-month-old boy hospitalized in intensive care revealed high gene expression and the complete genome of a novel isolate of KI polyomavirus (KIPyV). Further investigation detected minimal gene expression of additional viruses, suggesting that KIPyV was potentially the causal agent. Analysis of the complete genome of isolate NMKI001 revealed it is different from all previously reported genomes and contains two amino acid differences as compared to the closest virus isolate, Stockholm 380 (EF127908). J. Med. Virol. 89:926-930, 2017. © 2016 Wiley Periodicals, Inc.

Keywords: KI polyomavirus; complete genome; human polyomavirus 3; next generation sequencing; severe lower respiratory infection.

Publication types

  • Case Reports
  • Research Support, N.I.H., Extramural

MeSH terms

  • Cluster Analysis
  • Genome, Viral*
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Infant
  • Male
  • Phylogeny
  • Polyomavirus / genetics*
  • Polyomavirus / isolation & purification*
  • Polyomavirus Infections / virology*
  • Respiratory Tract Infections / virology*
  • Sequence Analysis, DNA*
  • Sequence Homology
  • Synteny