Bioactivity of Diterpens from the Ethyl Acetate Extract of Kingiodendron pinnatum Rox. Hams

Komal Kumar Javarappa; Attemode Girijanna Devi Prasad; A J Mahadesh Prasad; Chetana Mane

doi:10.4103/0974-8490.188871

Bioactivity of Diterpens from the Ethyl Acetate Extract of Kingiodendron pinnatum Rox. Hams

Pharmacognosy Res. 2016 Oct-Dec;8(4):287-291. doi: 10.4103/0974-8490.188871.

Authors

Komal Kumar Javarappa¹, Attemode Girijanna Devi Prasad², A J Mahadesh Prasad³, Chetana Mane⁴

Affiliations

¹ Department of Clinical and Experimental Medicine, Experimental Haematology Unit, University of Linkoping, Linkoping, Sweden.
² Department of Environmental Science, PBM Mahajana PG Centre, Mysore, Karnataka, India.
³ Department of Biochemistry, PBM Mahajana PG Centre, Mysore, Karnataka, India.
⁴ Department of Biochemistry, University of Mysore, Karnataka, India.

Abstract

Background: Kingiodendron pinnatum Rox. Hams. is an endangered medicinal plant used in gonorrhoe, catarrhal conditions of genito-urinary and respiratory tracts. The scientific and pharmacological formulation of K. pinnatum has not been established so far though it is being traditionally used by tribes of the region.

Objective: P hytochemical screening and identification of the bioactive compounds from the ethyl acetate extract of Kingiodendron pinnatum Rox. Hams.

Materials and methods: Chromatographic separation was carried out by thin layer chromatography and column chromatography. Bio-autography of the column fractioned extract and TLC chromatogram were evaluated in vitro for antibacterial activity. The PTLC, HP TLC were used for crude extract and HPLC, LCMS, FTIR, ¹HNMR and ¹³CNMR were employed for the isolated compound in the ethyl acetate extract of K. pinnatum.

Results: Evaluation of solvent system for chromatographic separation revealed that ethyl acetate: petroleum ether in the ratio of 7:2.5 ml was the most appropriate one for the separation of diterpene compounds. The antibacterial bio-autography screening of TLC separated compound showed positive activity with Staphylococcus aureus and negative activity with Escherichia coli. Spectroscopic analysis of the isolated compound from the ethyl acetate extract of K. pinnatum revealed the presence of diterpene compound.

Conclusion: It is evident from the present study that the ethyl acetate extract of K. pinnatum is rich in diterpene compounds and having potential antibacterial activity.

Summary: Novel extraction method for phytochemicls from Kingidendron pinnatum at RTAntibacterial property of diterpens extracted from Kingiodendron pinnatum Rox. Hams aganist S. aureus Abbreviations Used: TLC: Thin Layer Chromatography, PTLC: Preparatory Thin Layer Chromatography, HPTLC: High perormence Thin Layer chromatography, HPLC: High Performance Liquid Chromatography, LC-MS: Liquid chromatography Mass Spectra, FTIR: Fourier Transform Infrared Chromatography, NMR: Nuclear Magnetic Resonance.

Keywords: Bio-autography; HPLC; HPTLC; LC-MS; NMR.