Three new phenolic compounds (1-3), along with five known compounds (4-8) were isolated from the rhizome of Cimicifuga dahurica (Turcz.) Maxim. Their structures were elucidated by spectroscopic methods including 1D-NMR, 2D-NMR and HR-MS techniques. DPPH method and protective effect on PC12 cells against H2O2-induced oxidative damage model were carried to evaluate the antioxidant capability of these compounds. Compound 5 showed significant antioxidant activity with IC50 values 9.33μM in DPPH assay and compound 2 displayed marked neuro-protective effect with 87.65% cell viability at the concentration of 10μM. Additionally, the possible structure-activity relationships of these phenolic compounds were tentatively discussed.
Keywords: 1-O-feruloyl-β-d-glucopyranoside (PubChem CID: 13962928); 2-feruloyl piscidic acid (PubChem CID: 10002902); 2-isoferuloyl piscidic acid (PubChem CID: 6450179); 3,4-dimethoxycinnamic acid (PubChem CID: 717r1); Antioxidant; Carboxymethyl isoferulate (PubChem CID: 16729361); Cimicifuga dahurica (Turcz.) Maxim; Neuroprotective; Polyphenolic compounds.
Copyright © 2016 Elsevier B.V. All rights reserved.