Isothermal Amplification Strategies for Detection in Microfluidic Devices

Saheli Sarkar; Pooja Sabhachandani; Tania Konry

doi:10.1016/j.tibtech.2016.09.006

Isothermal Amplification Strategies for Detection in Microfluidic Devices

Trends Biotechnol. 2017 Mar;35(3):186-189. doi: 10.1016/j.tibtech.2016.09.006. Epub 2016 Sep 29.

Authors

Saheli Sarkar¹, Pooja Sabhachandani¹, Tania Konry²

Affiliations

¹ Department of Pharmaceutical Sciences, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA.
² Department of Pharmaceutical Sciences, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA. Electronic address: t.konry@neu.edu.

PMID: 27692899
DOI: 10.1016/j.tibtech.2016.09.006

Abstract

Isothermal rolling circle amplification (RCA) is used to detect nucleic and non-nucleic acid biomarkers with high sensitivity. Immuno-RCA, the specific detection of proteins via antigen-antibody recognition, has been miniaturized for microfluidic platforms to reduce reagent and sample consumption, accelerate reaction kinetics, and enhance the sensitivity and specificity of detection.

Keywords: droplets; immuno-rolling circle amplification; microfluidics; protein.

MeSH terms

Enzyme Assays / methods
Humans
Microfluidic Analytical Techniques / methods*
Nucleic Acid Amplification Techniques / methods*
Proteins / analysis
Single-Cell Analysis

Substances

Proteins