Transcriptional regulation analysis of FAM3A gene and its effect on adipocyte differentiation

Tingting Kang; Disha Peng; Guowei Bu; Hao Gu; Feng Zhang; Ruirui Zhang; Ying Zhou; Yuanzhu Xiong; Minggang Lei

doi:10.1016/j.gene.2016.09.038

Transcriptional regulation analysis of FAM3A gene and its effect on adipocyte differentiation

Gene. 2016 Dec 20;595(1):92-98. doi: 10.1016/j.gene.2016.09.038. Epub 2016 Sep 26.

Authors

Tingting Kang¹, Disha Peng¹, Guowei Bu¹, Hao Gu¹, Feng Zhang², Ruirui Zhang¹, Ying Zhou¹, Yuanzhu Xiong¹, Minggang Lei³

Affiliations

¹ Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
² Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, People's Republic of China.
³ Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: leimg@mail.hzau.edu.cn.

PMID: 27688071
DOI: 10.1016/j.gene.2016.09.038

Abstract

FAM3A (family with sequence similarity 3, member A) is regulated by PPARG and participates in the metabolism of lipid in liver. However, the transcriptional regulation analysis of FAM3A is very little and biological function of FAM3A still unclear. In this study, the core promoter region and transcription factor binding sites of FAM3A gene were identified and characterized using dual luciferase report experiments and electrophoretic mobility shift assays (EMSA). The promoter activity of FAM3A was dramatically decreased after the mutation of C/EBPβ binding sites, suggesting that C/EBPβ is a transcriptional activator of FAM3A. Overexpression of FAM3A significantly inhibited the efficiency of preadipocytes to differentiate into adipocytes as indicated by Western Blot and Oil Red O staining assay. These results suggest that C/EBPβ plays an important role in regulating FAM3A promoter activity and FAM3A inhibits adipocyte differentiation.

Keywords: Adipogenesis; C/EBPβ; FAM3A; Promoter.

MeSH terms

3T3-L1 Cells
Adipocytes / metabolism*
Animals
CCAAT-Enhancer-Binding Protein-beta / genetics
CCAAT-Enhancer-Binding Protein-beta / metabolism
CHO Cells
Cell Differentiation / physiology*
Cricetinae
Cricetulus
Cytokines / biosynthesis*
Cytokines / genetics
Humans
Mice
Mutation
Response Elements / physiology*
Transcription, Genetic / physiology*

Substances

CCAAT-Enhancer-Binding Protein-beta
CEBPB protein, human
Cytokines
FAM3A protein, human