Novel mononuclear Pt2+ and Pd2+ complexes containing (2,3-f)pyrazino(1,10)phenanthroline-2,3-dicarboxylic acid as a multi-donor ligand. Synthesis, structure, interaction with DNA, in vitro cytotoxicity, and apoptosis

Ya-Guang Sun; Yi-Ning Sun; Li-Xin You; Yi-Nan Liu; Fu Ding; Bao-Yi Ren; Gang Xiong; Valerian Dragutan; Ileana Dragutan

doi:10.1016/j.jinorgbio.2016.09.008

Novel mononuclear Pt²⁺ and Pd²⁺ complexes containing (2,3-f)pyrazino(1,10)phenanthroline-2,3-dicarboxylic acid as a multi-donor ligand. Synthesis, structure, interaction with DNA, in vitro cytotoxicity, and apoptosis

J Inorg Biochem. 2016 Nov:164:129-140. doi: 10.1016/j.jinorgbio.2016.09.008. Epub 2016 Sep 14.

Authors

Ya-Guang Sun¹, Yi-Ning Sun², Li-Xin You², Yi-Nan Liu³, Fu Ding², Bao-Yi Ren², Gang Xiong⁴, Valerian Dragutan⁵, Ileana Dragutan⁶

Affiliations

¹ The Key Laboratory of the Inorganic Molecule-Based Chemistry of Liaoning Province and Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China. Electronic address: sunyaguang@syuct.edu.cn.
² The Key Laboratory of the Inorganic Molecule-Based Chemistry of Liaoning Province and Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China.
³ Life Engineering College, Shenyang Institute of Technology, Fushun 113122, China.
⁴ The Key Laboratory of the Inorganic Molecule-Based Chemistry of Liaoning Province and Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China; Department of Chemistry, Nankai University, Tianjin 300007,China.
⁵ Institute of Organic Chemistry "C.D. Nenitescu", Romanian Academy, 060023 Bucharest, Romania.
⁶ Institute of Organic Chemistry "C.D. Nenitescu", Romanian Academy, 060023 Bucharest, Romania. Electronic address: idragutan@yahoo.com.

PMID: 27670262
DOI: 10.1016/j.jinorgbio.2016.09.008

Abstract

New cytotoxic, mononuclear Pt and Pd coordination complexes featuring the planar, multi-donor ligand (2,3-f)pyrazino(1,10)phenanthroline-2,3-dicarboxylic acid (H₂PPDA) have been synthesized under hydrothermal conditions (in water, at high temperature and pressure) and fully characterized. The complexes were proven to be isostructural by applying the single-crystal X-ray diffraction technique. UV-Vis and fluorescence spectroscopy investigations on their interaction with fish sperm DNA revealed a considerable binding capacity while gel electrophoresis provided evidence in favor of cleavage of pBR322 plasmid DNA. The newly synthesized complexes manifested significant in vitro cytotoxic activity against two different human cancer cell lines, the KB and JEKO cells, with cell death mainly caused by apoptosis.

Keywords: Apoptosis; Cytotoxicity; DFT calculations; Phenanthroline ligands; Pt(2+) and Pd(2+) coordination complexes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis / drug effects*
Cell Line, Tumor
Coordination Complexes* / chemical synthesis
Coordination Complexes* / chemistry
Coordination Complexes* / pharmacology
Cytotoxins* / chemical synthesis
Cytotoxins* / chemistry
Cytotoxins* / pharmacology
DNA / chemistry*
Humans
Palladium* / chemistry
Palladium* / pharmacology
Plasmids / chemistry*
Platinum* / chemistry
Platinum* / pharmacology

Substances

Coordination Complexes
Cytotoxins
Platinum
Palladium
DNA