Objective: To explore the application value of assisted laparoscopic ultrasound in myomectomy. Methods: One hundred and fifty six patients who underwent myomectomy in Beijing Obstetrics and Gynecology Hospital from 2011 to 2013 were enrolled.The patients were randomly divided into laparoscopic ultrasound guided group (n=57), laparoscopic group (n=54) and open resection group (n=45). The patients were followed up to calculate the residue and recurrence rates of uterine fibroids by vaginal ultrasound at 6th month and 12th-18th month after the operation. The residual and recurrence rates of the three methods were calculated and compared. Results: The recurrence rate of laparoscopic ultrasound guided group wassignificantly lower than that of laparoscopic group and open resection group (P<0.01) and (P<0.01). The residual rate of laparoscopic ultrasound guided group was also lower than that of laparoscopic group(P<0.05). However, there was no difference between the residual rates of laparoscopic ultrasound guided group and open resection group (P>0.05). When compared with open resection group, no difference was found in the residual and recurrence rates of laparoscopic group. The number of myoma was proportional to the residual rate and the recurrence rate. When the number of the myoma was more than 10, most of the cases existed residue. Conclusion: The application of assisted laparoscopic ultrasound in myomectomy couldreduce the residual and recurrence rate of uterine fibroids.