Detection and counting of a submicrometer particle in liquid flow by self-mixing microchip Yb:YAG laser velocimetry

Appl Opt. 2016 Sep 20;55(27):7574-82. doi: 10.1364/AO.55.007574.

Abstract

We observed intermittent modulation by scattered light from a single submicrometer particle moving in the flow channel using a self-mixing microchip Yb:YAG laser Doppler velocimeter (LDV) under lateral beam access. The Doppler-shift frequency chirping (i.e., velocity change) was identified in accordance with a particle passage through the beam focus. Single particle counting, which obeys the Poisson distribution, was performed successfully over a long period of time. The experimental results have been reproduced by a numerical simulation. The LDV signal was increased over 20 dB for a 202-nm particle without chirping by collinear beam access with the laser beam axis aligned along the flow direction.