Biological and molecular characterization of a tomato isolate of potato virus Y (PVY) of the PVYC lineage

Mohamad Chikh-Ali; Dalton Vander Pol; Olga V Nikolaeva; Michael J Melzer; Alexander V Karasev

doi:10.1007/s00705-016-3071-9

Biological and molecular characterization of a tomato isolate of potato virus Y (PVY) of the PVY^C lineage

Arch Virol. 2016 Dec;161(12):3561-3566. doi: 10.1007/s00705-016-3071-9. Epub 2016 Sep 21.

Authors

Mohamad Chikh-Ali¹, Dalton Vander Pol¹, Olga V Nikolaeva¹, Michael J Melzer², Alexander V Karasev^{3

4}

Affiliations

¹ Department of PSES, University of Idaho, Moscow, ID, 83844-2339, USA.
² Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, HI, USA.
³ Department of PSES, University of Idaho, Moscow, ID, 83844-2339, USA. akarasev@uidaho.edu.
⁴ Bioinformatics and Computational Biology Program, University of Idaho, Moscow, ID, USA. akarasev@uidaho.edu.

PMID: 27654668
DOI: 10.1007/s00705-016-3071-9

Abstract

An isolate of potato virus Y (PVY), PVY-H14, was collected on the island of Oahu, Hawaii, from tomato plants exhibiting stunting and necrotic lesions on leaves. PVY-H14 triggered the hypersensitive resistance response in potato cultivars King Edward and Maris Bard, typical of a PVY^C strain, and was unable to infect systemically the four tested cultivars, Desiree, Maris Bard, King Edward, and Russet Norkotah. Phylogenetic analysis of H14 and the whole genomes of 31 PVY isolates of non-recombinant strains of PVY placed PVY-H14 in the same clade with PVY^C and several unclassified PVY isolates from tomato and tobacco.

MeSH terms

Cluster Analysis
Genome, Viral
Genotype
Hawaii
Phylogeny
Plant Diseases / virology*
Plant Leaves / virology
Potyvirus / classification
Potyvirus / genetics
Potyvirus / isolation & purification*
Potyvirus / pathogenicity*
RNA, Viral / genetics
Sequence Analysis, DNA
Sequence Homology
Solanum lycopersicum / virology*

Substances

RNA, Viral