Isolation and characterization of an acidic lethal phospholipase Az from Malayan cobra (Naja naja sputatrix) venom

Biochem Int. 1989 Apr;18(4):785-92.

Abstract

An acidic, lethal phospholipase Az was purified to electrophoretic homogeneity from the venom of the Malayan cobra (Naja naja sputatrix). The enzyme has an isoelectric point of 5.58, a molecular weight of 12000, and a medium lethal dose (LD50) of 0.86 micrograms/g in mice by intravenous injection. The enzyme also exhibited weak anticoagulant and edema-forming activities. The amino acid composition of the enzyme is similar to those of other cobra venom phospholipases Az.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / analysis
  • Animals
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Elapid Venoms / analysis*
  • Hydrogen-Ion Concentration
  • Lethal Dose 50
  • Mice
  • Molecular Weight
  • Phospholipases / isolation & purification*
  • Phospholipases A / isolation & purification*
  • Phospholipases A / toxicity
  • Snakes

Substances

  • Amino Acids
  • Elapid Venoms
  • Phospholipases
  • Phospholipases A