Capillary Electrophoresis in Food and Foodomics

Methods Mol Biol. 2016:1483:471-507. doi: 10.1007/978-1-4939-6403-1_22.

Abstract

Quality and safety assessment as well as the evaluation of other nutritional and functional properties of foods imply the use of robust, efficient, sensitive, and cost-effective analytical methodologies. Among analytical technologies used in the fields of food analysis and foodomics, capillary electrophoresis (CE) has generated great interest for the analyses of a large number of compounds due to its high separation efficiency, extremely small sample and reagent requirements, and rapid analysis. The introductory section of this chapter provides an overview of the recent applications of capillary electrophoresis (CE) in food analysis and foodomics. Relevant reviews and research articles on these topics are tabulated including papers published in the period 2011-2014. In addition, to illustrate the great capabilities of CE in foodomics the chapter describes the main experimental points to be taken into consideration for a metabolomic study of the antiproliferative effect of carnosic acid (a natural diterpene found in rosemary) against HT-29 human colon cancer cells.

Keywords: CE-MS; Capillary electrophoresis; Food bioactivity; Food quality; Food safety; Food traceability; Foodomics; Metabolomics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophoresis, Capillary / methods*
  • Food Analysis / methods*
  • HT29 Cells
  • Humans
  • Mass Spectrometry / methods
  • Metabolomics / methods*
  • Plant Extracts / chemistry
  • Plant Extracts / isolation & purification

Substances

  • Plant Extracts