Stable isotope dimethyl labelling for quantitative proteomics and beyond

Jue-Liang Hsu; Shu-Hui Chen

doi:10.1098/rsta.2015.0364

Stable isotope dimethyl labelling for quantitative proteomics and beyond

Philos Trans A Math Phys Eng Sci. 2016 Oct 28;374(2079):20150364. doi: 10.1098/rsta.2015.0364.

Authors

Jue-Liang Hsu¹, Shu-Hui Chen²

Affiliations

¹ Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung, Taiwan, Republic of China jlhsu@mail.npust.edu.tw.
² Department of Chemistry, National Cheng Kung University, Tainan City, Taiwan, Republic of China shchen@mail.ncku.edu.tw.

Abstract

Stable-isotope reductive dimethylation, a cost-effective, simple, robust, reliable and easy-to- multiplex labelling method, is widely applied to quantitative proteomics using liquid chromatography-mass spectrometry. This review focuses on biological applications of stable-isotope dimethyl labelling for a large-scale comparative analysis of protein expression and post-translational modifications based on its unique properties of the labelling chemistry. Some other applications of the labelling method for sample preparation and mass spectrometry-based protein identification and characterization are also summarized.This article is part of the themed issue 'Quantitative mass spectrometry'.

Keywords: LC-MS/MS; dimethyl labelling; post-translational modifications; quantitative proteomics; stable-isotope labelling.

Publication types

Review

MeSH terms

Animals
Chromatography, Liquid
Humans
Isotope Labeling / methods*
Mass Spectrometry
Methylation
Protein Processing, Post-Translational
Proteomics / methods*