Electrooptical Analysis of Microbial Cell Suspensions forDetermination of Antibiotic Resistance

Cell Biochem Biophys. 2016 Dec;74(4):537-544. doi: 10.1007/s12013-016-0762-5. Epub 2016 Sep 16.

Abstract

The effects of ampicillin; kanamycin, chloramphenicol, and tetracycline on electrophysical characteristics of cells of sensitive (ampicillin; kanamycin, chloramphenicol) and resistant (ampicillin; kanamycin, chloramphenicol, tetracycline) Escherichia coli strains were studied. Under the action of antibiotics sensitive and resistant E. coli strains acquire different electro-optical properties. Changes in suspension-orientational spectra, that are observed under the action of ampicillin; kanamycin, chloramphenicol, and tetracycline can be used in determination of antibiotic resistance of the studied bacterial strains. In our opinion, the methods of microbial suspension electro-optical analysis can be used in microbiology, mеdicinе, veterinary, and are an effective tool for solving the problems connected with determination of microbial cell antibiotic resistance.

Keywords: Ampicillin; Cells orientational spectrum; Chloramphenicol; Escherichia coli; Kanamycin; Tetracycline.

MeSH terms

  • Ampicillin / pharmacology
  • Anti-Bacterial Agents / pharmacology*
  • Cell Polarity / drug effects
  • Chloramphenicol / pharmacology
  • Drug Resistance, Microbial
  • Electricity
  • Escherichia coli / drug effects*
  • Escherichia coli / physiology
  • Kanamycin / pharmacology
  • Light
  • Scattering, Radiation
  • Tetracycline / pharmacology

Substances

  • Anti-Bacterial Agents
  • Kanamycin
  • Chloramphenicol
  • Ampicillin
  • Tetracycline