Electrophysiological approaches to unravel the neurobiological basis of appetite and satiety: use of the multielectrode array as a screening strategy

Abstract

Hypothalamic neural circuits are recognised as primary sites of the neuromodulator effect of homeostatic food intake, whereas changes in ventral tegmental area (VTA), hippocampus and amygdala have been implicated in the hedonic, cognitive and emotional aspects of eating. Here, we discuss synaptic transmission and plasticity within brain circuits governing appetite and food intake behaviour, focusing on the metabolic hormones ghrelin and leptin. We discuss functional changes within these circuitries and critically assess the applicability of electrophysiological measurements using in vitro multielectrode array (MEA) systems to identify novel appetite modulators. Stringent validation of functional assays to screen neuroactive substrates is of crucial importance for the discovery of novel food intake modulators, with major implications for the nutraceutical food industry and drug development.