NMR study of Met-1 human Angiogenin: (1)H, (13)C, (15)N backbone and side-chain resonance assignment

Aikaterini C Tsika; Demetra S M Chatzileontiadou; Demetres D Leonidas; Georgios A Spyroulias

doi:10.1007/s12104-016-9704-9

NMR study of Met-1 human Angiogenin: (1)H, (13)C, (15)N backbone and side-chain resonance assignment

Biomol NMR Assign. 2016 Oct;10(2):379-83. doi: 10.1007/s12104-016-9704-9. Epub 2016 Sep 13.

Authors

Aikaterini C Tsika¹, Demetra S M Chatzileontiadou², Demetres D Leonidas³, Georgios A Spyroulias⁴

Affiliations

¹ Department of Pharmacy, University of Patras, 26504, Patras, Greece.
² Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, 41500, Larissa, Greece.
³ Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, 41500, Larissa, Greece. ddleonidas@bio.uth.gr.
⁴ Department of Pharmacy, University of Patras, 26504, Patras, Greece. G.A.Spyroulias@upatras.gr.

PMID: 27624767
DOI: 10.1007/s12104-016-9704-9

Abstract

Here, we report the high yield expression and preliminary structural analysis via solution hetero-nuclear NMR spectroscopy of the recombinant Met-1 human Angiogenin. The analysis reveals a well folded as well as, a monomeric polypeptide. Τhe sequence-specific assignment of its (1)H, (15)N and (13)C resonances at high percentage was obtained. Also, using TALOS+ its secondary structure elements were determined.

Keywords: Angiogenesis; Angiogenin; NMR spectroscopy; RNase superfamily; Recombinant protein expression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Nuclear Magnetic Resonance, Biomolecular*
Ribonuclease, Pancreatic / chemistry*

Substances

angiogenin
Ribonuclease, Pancreatic