Ecophysiology matters: linking inorganic carbon acquisition to ecological preference in four species of microalgae (Chlorophyceae)

Abstract

The effect of CO₂ supply is likely to play an important role in algal ecology. Since inorganic carbon (C_i ) acquisition strategies are very diverse among microalgae and C_i availability varies greatly within and among habitats, we hypothesized that C_i acquisition depends on the pH of their preferred natural environment (adaptation) and that the efficiency of C_i uptake is affected by CO₂ availability (acclimation). To test this, four species of green algae originating from different habitats were studied. The pH-drift and C_i uptake kinetic experiments were used to characterize C_i acquisition strategies and their ability to acclimate to high and low CO₂ conditions and high and low pH was evaluated. Results from pH drift experiments revealed that the acidophile and acidotolerant Chlamydomonas species were mainly restricted to CO₂ , whereas the two neutrophiles were efficient bicarbonate users. CO₂ compensation points in low CO₂ -acclimated cultures ranged between 0.6 and 1.4 μM CO₂ and acclimation to different culture pH and CO₂ conditions suggested that CO₂ concentrating mechanisms were present in most species. High CO₂ acclimated cultures adapted rapidly to low CO₂ condition during pH-drifts. C_i uptake kinetics at different pH values showed that the affinity for C_i was largely influenced by external pH, being highest under conditions where CO₂ dominated the C_i pool. In conclusion, C_i acquisition was highly variable among four species of green algae and linked to growth pH preference, suggesting that there is a connection between C_i acquisition and ecological distribution.

Keywords: CCM; Chlamydomonas; Chlorella; Scenedesmus; CO2 supply; acidophile; carbon acquisition; extremophile; inorganic carbon uptake kinetics; pH-drift.