Customized sequential multiplex PCR for accurate and early determination of invasive pneumococcal serotypes found in India

J Microbiol Methods. 2016 Nov:130:133-135. doi: 10.1016/j.mimet.2016.09.007. Epub 2016 Sep 10.

Abstract

For accurate and earlier detection of invasive pneumococcal serogroup/serotypes from India, we have rearranged the African sequence of multiplex PCR provided by the Centers for Disease Control and Prevention, USA. This modified approach can successfully be adapted for earlier serotype detection of 95% of the pneumococcal strains prevalent in India.

Keywords: Customized sequential multiplex PCR; Indian serotypes; Invasive pneumococcal disease; PCV; Pneumococcal serotyping.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agglutination Tests / methods
  • Bacterial Proteins / genetics
  • Base Sequence
  • DNA, Bacterial / analysis
  • Early Diagnosis
  • Humans
  • India
  • Introduced Species
  • Multiplex Polymerase Chain Reaction / methods*
  • Pneumococcal Infections / blood
  • Pneumococcal Infections / cerebrospinal fluid
  • Pneumococcal Infections / diagnosis*
  • Pneumococcal Infections / microbiology*
  • Prevalence
  • Serogroup
  • Serotyping / methods*
  • Streptococcus pneumoniae / genetics*
  • Streptococcus pneumoniae / immunology
  • Streptococcus pneumoniae / isolation & purification*
  • Streptococcus pneumoniae / pathogenicity

Substances

  • Bacterial Proteins
  • CpsA protein, Streptococcus pneumoniae
  • DNA, Bacterial