Bone formation of human mesenchymal stem cells harvested from reaming debris is stimulated by low-dose bone morphogenetic protein-7 application in vivo

Fabian Westhauser; Melanie Höllig; Bruno Reible; Kai Xiao; Gerhard Schmidmaier; Arash Moghaddam

doi:10.1016/j.jor.2016.08.002

Bone formation of human mesenchymal stem cells harvested from reaming debris is stimulated by low-dose bone morphogenetic protein-7 application in vivo

J Orthop. 2016 Aug 29;13(4):404-8. doi: 10.1016/j.jor.2016.08.002. eCollection 2016 Dec.

Authors

Fabian Westhauser¹, Melanie Höllig¹, Bruno Reible¹, Kai Xiao¹, Gerhard Schmidmaier¹, Arash Moghaddam¹

Affiliation

¹ HTRG - Heidelberg Trauma Research Group, Trauma and Reconstructive Surgery, Center of Orthopedics, Traumatology, and Spinal Cord Injury, Heidelberg University Hospital, Schlierbacher Landstraße 200a, D-69118 Heidelberg, Germany.

Abstract

Stimulation of mesenchymal stem cells (MSC) by bone morphogenetic protein-7 (BMP-7) leads to superior bone formation in vitro. In this in vivo-study we evaluated the use of BMP-7 in combination with MSC isolated from reaming debris (RIA-MSC) and iliac crest bone marrow (BMSC) with micro-computed tomography (mCT)-analysis. β-Tricalciumphosphate scaffolds coated with BMSC and RIA-MSC were stimulated with three different BMP-7-concentrations and implanted ectopically in severe combined immunodeficiency (SCID) mice. Our results demonstrate that RIA-MSC show a higher osteogenic potential in vivo compared to BMSC. Ossification increased in direct correlation with the BMP-7-dose applied, however low-dose-stimulation by BMP-7 was more effective for RIA-MSC.

Keywords: Bone morphogenetic protein-7 (BMP-7); Human mesenchymal stem cells (MSC); Micro-computed tomography (mCT); Reaming material; Tissue engineering.