Formulation and characterization of esterified xylo-oligosaccharides-stabilized oil-in-water emulsions using microchannel emulsification

Sunsanee Udomrati; Nauman Khalid; Shoichi Gohtani; Mitsutoshi Nakajima; Kunihiko Uemura; Isao Kobayashi

doi:10.1016/j.colsurfb.2016.09.003

Formulation and characterization of esterified xylo-oligosaccharides-stabilized oil-in-water emulsions using microchannel emulsification

Colloids Surf B Biointerfaces. 2016 Dec 1:148:333-342. doi: 10.1016/j.colsurfb.2016.09.003. Epub 2016 Sep 6.

Authors

Sunsanee Udomrati¹, Nauman Khalid², Shoichi Gohtani³, Mitsutoshi Nakajima⁴, Kunihiko Uemura⁵, Isao Kobayashi⁶

Affiliations

¹ Food Research Institute, NARO, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan; Institute of Food Research and Product Development, Kasetsart University, 50 Chatuchak, Bangkok 10900, Thailand.
² Food Research Institute, NARO, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan; The Alliance for Research on North Africa, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan; School of Food and Agricultural Sciences, University of Management and Technology, Lahore 54000, Pakistan.
³ Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, 2393 Miki, Kagawa 761-0795, Japan.
⁴ Food Research Institute, NARO, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan; The Alliance for Research on North Africa, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan.
⁵ Food Research Institute, NARO, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan; The Alliance for Research on North Africa, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan.
⁶ Food Research Institute, NARO, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan; The Alliance for Research on North Africa, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan. Electronic address: isaok@affrc.go.jp.

PMID: 27616069
DOI: 10.1016/j.colsurfb.2016.09.003

Abstract

A series of amphiphilically esterified xylo-oligosaccharides (xylo esters) with different fatty acids residues - decanoic acid (C-10), lauric acid (C-12) and palmitic acid (C-16) - were enzymatically modified at 60°C for 4h. These xylo esters were used as emulsifiers to formulate oil-in-water (O/W) emulsions by microchannel emulsification (MCE). Grooved and straight-through MCE was used to investigate the droplet generation and/or emulsion stability. Xylo ester-stabilized oil droplets were generated smoothly from microchannels arranged linearly or two dimensionally, while xylo ester-stabilized emulsions were less monodispersed owing to low surface activity of the xylo esters. The combined use of xylo esters (2.5% (w/w)) and Tween series (0.1% (w/w)) in the continuous phase can improve the monodispersity of the resultant oil. Successful droplet generation was achieved with the straight-through MCE using 2.5% (w/w) xylo laurate and 0.1% (w/w) Tween 20. The optimized combination of xylo laurate and Tween 20 inhibited coalescence and oiling off more efficiently than the droplets solely stabilized by Tween 20 during 30days of storage.

Keywords: Fatty acid ester; Microchannel emulsification; Oil-in-water emulsion; Stability; Xylo-oligosaccharide.

MeSH terms

Emulsions*
Esterification
Oils
Oligosaccharides / chemistry*
Water

Substances

Emulsions
Oils
Oligosaccharides
Water