Microbial responses to various process disturbances in a continuous hydrogen reactor fed with galactose

Gopalakrishnan Kumar; Jeong-Hoon Park; Periyasamy Sivagurunathan; Sang-Hoon Lee; Hee-Deung Park; Sang-Hyoun Kim

doi:10.1016/j.jbiosc.2016.08.006

Microbial responses to various process disturbances in a continuous hydrogen reactor fed with galactose

J Biosci Bioeng. 2017 Feb;123(2):216-222. doi: 10.1016/j.jbiosc.2016.08.006. Epub 2016 Sep 7.

Authors

Gopalakrishnan Kumar¹, Jeong-Hoon Park², Periyasamy Sivagurunathan³, Sang-Hoon Lee⁴, Hee-Deung Park², Sang-Hyoun Kim⁵

Affiliations

¹ Sustainable Management of Natural Resources and Environment Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Viet Nam; Department of Environmental Engineering, Daegu University, Gyeongsan, Gyeongbuk 712-714, South Korea.
² School of Civil, Environmental and Architectural Engineering, Korea University, Anam-Dong, Seongbuk-gu, Seoul 136-714, South Korea.
³ Department of Environmental Engineering, Daegu University, Gyeongsan, Gyeongbuk 712-714, South Korea.
⁴ School of Civil, Environmental and Architectural Engineering, Korea University, Anam-Dong, Seongbuk-gu, Seoul 136-714, South Korea; Department of Microbiology & Molecular Genetics, Michigan State University, East Lansing, MI 48824-4320, USA.
⁵ Department of Environmental Engineering, Daegu University, Gyeongsan, Gyeongbuk 712-714, South Korea. Electronic address: sanghkim1@daegu.ac.kr.

PMID: 27614820
DOI: 10.1016/j.jbiosc.2016.08.006

Abstract

In this study, microbial responses of a continuous hydrogen reactor fed with galactose have been investigated. Process disturbances reduced H₂ production performance as well as large fluctuations in microbial diversity. The peak values of the hydrogen yield (HY) was not influenced greatly during the steady state period, and accounted as 2.01 ± 0.05 and 2.14 ± 0.03 mol/mol galactose_added, while hydraulic retention time (HRT) was at 12 and 8 h, respectively. Microbial community analysis via 454 pyrosequencing revealed that functional redundancy following changes in the microbial community distribution led to the stability of the fermentation performance. The butyrate to acetate (B/A) ratio well correlated with changes in the microbial community. The energy generation rate and energy yield resulted in the peak values of 134 kJ/L-d and 612 kJ/mol_added.

Keywords: 454 Pyrosequencing; Biohydrogen; Energy generation rate; Functional redundancy; Microbial consortia.

MeSH terms

Adaptation, Biological* / drug effects
Adaptation, Biological* / genetics
Bioelectric Energy Sources / microbiology
Biofuels
Bioreactors / microbiology*
Butyric Acid / metabolism
Fermentation / drug effects
Fermentation / genetics
Galactose / metabolism
Galactose / pharmacology*
Hydrogen / chemistry
Hydrogen / metabolism*
Microbial Consortia / drug effects
Microbial Consortia / genetics*
Sequence Analysis, DNA / methods

Substances

Biofuels
Butyric Acid
Hydrogen
Galactose