Evaluation of a Multiplex PCR Assay To Rapidly Detect Enterobacteriaceae with a Broad Range of β-Lactamases Directly from Perianal Swabs

Antimicrob Agents Chemother. 2016 Oct 21;60(11):6957-6961. doi: 10.1128/AAC.01458-16. Print 2016 Nov.

Abstract

We developed and evaluated multiplexed molecular beacon probes in a real-time PCR assay to identify prominent extended-spectrum-β-lactamase, plasmid-mediated AmpC β-lactamase (pAmpC) and carbapenemase genes directly from perianal swab specimens within 6 h. We evaluated this assay on 158 perianal swabs collected from hematopoietic stem cell transplant recipients and found that this assay was highly sensitive and specific for detection of CTX-M-, pAmpC-, and KPC-producing Enterobacteriaceae compared to culture on chromogenic agar.

Publication types

  • Evaluation Study

MeSH terms

  • Anal Canal / microbiology*
  • Bacterial Proteins / genetics
  • Enterobacteriaceae / genetics*
  • Enterobacteriaceae / isolation & purification
  • Escherichia coli Proteins / genetics
  • Hematopoietic Stem Cell Transplantation
  • Humans
  • Multiplex Polymerase Chain Reaction / methods*
  • Specimen Handling / methods
  • beta-Lactamases / genetics*

Substances

  • Bacterial Proteins
  • Escherichia coli Proteins
  • AmpC beta-lactamases
  • beta-Lactamases
  • beta-lactamase CTX-M, E coli
  • carbapenemase