Complete Sequencing of Plasmids Containing blaOXA-163 and blaOXA-48 in Escherichia coli Sequence Type 131

Nicole Stoesser; Anna E Sheppard; Gisele Peirano; Robert Sebra; Tarah Lynch; Luke Anson; Andrew Kasarskis; Mary R Motyl; Derrick W Crook; Johann D Pitout

doi:10.1128/AAC.01130-16

Complete Sequencing of Plasmids Containing blaOXA-163 and blaOXA-48 in Escherichia coli Sequence Type 131

Antimicrob Agents Chemother. 2016 Oct 21;60(11):6948-6951. doi: 10.1128/AAC.01130-16. Print 2016 Nov.

Authors

Nicole Stoesser¹, Anna E Sheppard², Gisele Peirano^{3

4}, Robert Sebra⁵, Tarah Lynch⁴, Luke Anson², Andrew Kasarskis⁵, Mary R Motyl⁶, Derrick W Crook², Johann D Pitout^{3

4

7

8

9}

Affiliations

¹ Modernizing Medical Microbiology Consortium, Nuffield Department of Medicine, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom nicole.stoesser@ndm.ox.ac.uk.
² Modernizing Medical Microbiology Consortium, Nuffield Department of Medicine, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom.
³ Division of Microbiology, Calgary Laboratory Services, Calgary, Alberta, Canada.
⁴ Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada.
⁵ Icahn Institute and Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
⁶ Clinical Microbiology, Merck and Co., Inc., Rahway, New Jersey, USA.
⁷ Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada.
⁸ Snyder Institute for Chronic Diseases, University of Calgary, Calgary, Alberta, Canada.
⁹ Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa.

Abstract

OXA-48-like enzymes have emerged as important extended-spectrum β-lactamases/carbapenemases in Escherichia coli sequence type 131 (ST131). We report the structures of the first fully sequenced bla_OXA-163 plasmid and of two other bla_OXA-48 plasmids in this lineage. bla_OXA-163 was located on a 71-kb IncN plasmid with other resistance genes. bla_OXA-48 was present on IncL/M plasmids, genetically similar to other bla_OXA-48 plasmid sequences, and consistent with interspecies/interlineage spread. The presence of bla_OXA-48-like genes on epidemic plasmids in ST131 is of concern.

MeSH terms

Drug Resistance, Bacterial / genetics
Escherichia coli / drug effects
Escherichia coli / genetics*
Escherichia coli Proteins / genetics*
Escherichia coli Proteins / metabolism
Microbial Sensitivity Tests
Plasmids / genetics
beta-Lactamases / genetics*
beta-Lactamases / metabolism

Substances

Escherichia coli Proteins
beta-Lactamases
beta-lactamase OXA-163, E coli
beta-lactamase OXA-48, E coli

Abstract

MeSH terms

Substances

Grants and funding