The Immunogenicity of Peptoid-Protein Conjugates

Allison Case; Angela Desmond; Daniel Lopes; Kelly Dye; Kelly Mapes; Stephen Ruback; Iliodora Pop; Jiyeun Kate Kim; Pavitra Chakravarty; Joan E Smallshaw; Laurentiu M Pop; Ellen S Vitetta

doi:10.4172/2157-7560.1000329

The Immunogenicity of Peptoid-Protein Conjugates

J Vaccines Vaccin. 2016 Aug;7(4):329. doi: 10.4172/2157-7560.1000329. Epub 2016 Jul 11.

Authors

Affiliations

¹ Cancer Immunobiology Center and Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Department of Bioengineering, The University of Texas at Dallas, Texas, USA.
² Cancer Immunobiology Center and Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Department of Pediatrics, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.
³ Cancer Immunobiology Center and Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Eli Lilly, Indianapolis, Indiana, USA.
⁴ Cancer Immunobiology Center and Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, USA; School of Medicine, Texas Tech University Health Sciences Center, Lubbock, Texas, USA.
⁵ Cancer Immunobiology Center and Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Green Center for Systems Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
⁶ Cancer Immunobiology Center and Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
⁷ Cancer Immunobiology Center and Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
⁸ Cancer Immunobiology Center and Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, USA; College of Pharmacy, Pusan National University, Busan, Republic of Korea.
⁹ Cancer Immunobiology Center and Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
¹⁰ Cancer Immunobiology Center and Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.

Abstract

We demonstrate that a peptoid composed of five monomers and attached via a maleimide linker to a carrier protein elicits anti-peptoid, anti-linker and anti-carrier antibodies in rabbits. Specific anti-peptoid antibodies were affinity purified and used to reproducibly retrieve three specific peptoid-coupled beads from 20,000 irrelevant peptoid-beads using magnetic screening.

Abstract

Grants and funding