Objective: To test the expression of autoantibody against the M2-muscarinic receptor in patients with severe preeclampsia.
Methods: A case-control study, including 78 patients with severe preeclampsia and 78 women with normal pregnancy in Department of Gynaecology and Obstetrics of Capital Medical University affiliated Beijing Chao-Yang Hospital from Jan, 2013 to Jan, 2015, were conducted, and 60 non-pregnant women were served as a control group. ELISA protocol was used to test serum autoantibody against the M2-muscarinic receptor. The clinical significance of the autoantibody against the M2-muscarinic receptor among women with severe preeclampsia was estimated.
Results: Autoantibody against the M2-muscarinic receptor were positive in 32.1% (25/78) of patients with severe preeclampsia, in 10.3% (8/78, P<0.05) of normal pregnant women and in 8.3% (5/60, P<0.05) of non-pregnant controls. The concentration of creatine kinase in patients with severe preeclampsia and normal pregnant women were (101.49±142.75) and (57.94±31.64) U/L, respectively. Mask difference exist between the severe preeclampsia group and the normal pregnancy group (P<0.05).
Conclusion: The expression of autoantibody against the M2-muscarinic receptor in patients with severe preeclampsia is elevated significantly, which is associated with severe preeclampsia. However, its etiological role needs further to be investigated.
目的：检测M2受体自身抗体在重度子痫前期患者中的表达，探讨其临床意义。方法：选择2013年1月至2015年1月在首都医科大学附属朝阳医院妇产科收治的78例重度子痫前期孕妇为研究对象，并取同期收治的78例正常单胎足月孕妇和60例未妊娠妇女作为对照。采用ELISA方法检测3组妇女血浆中的M2受体自身抗体水平，并分析其在重度子痫前期中的临床意义。结果：重度子痫患者M2受体自身抗体阳性率32.1%(25/78)，正常妊娠组为10.3%(8/78)，两组阳性率比较差异有统计学意义(P<0.01)；未妊娠组阳性率8.3%(5/60)，与正常妊娠组比较差异无统计学意义(P>0.05)；此外，重度子痫前期患者血清中肌酸激酶浓度为(101.49±142.75) U/L，而正常妊娠组肌酸激酶含量为(57.94.±31.64) U/L，两组比较差异有统计学意义(P<0.05)。结论：M2受体自身抗体在重度子痫前期患者中的表达明显增高，可能与其发病有关。.