A naphthalene-based two-photon fluorescent probe for selective and sensitive detection of endogenous hypochlorous acid

Xiao-Hong Zhou; Yu-Ren Jiang; Xiong-Jie Zhao; Dong Guo

doi:10.1016/j.talanta.2016.07.047

A naphthalene-based two-photon fluorescent probe for selective and sensitive detection of endogenous hypochlorous acid

Talanta. 2016 Nov 1:160:470-474. doi: 10.1016/j.talanta.2016.07.047. Epub 2016 Jul 27.

Authors

Xiao-Hong Zhou¹, Yu-Ren Jiang², Xiong-Jie Zhao³, Dong Guo³

Affiliations

¹ College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083 China; Environment Monitoring Department, Changsha Environmental Protection College, Changsha, 410004 China.
² College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083 China. Electronic address: jiangyr@mail.csu.edu.cn.
³ College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083 China.

PMID: 27591640
DOI: 10.1016/j.talanta.2016.07.047

Abstract

An efficient naphthalene-based two-photon fluorescent probe for endogenous HClO has been reported in the present study, which consists of a 6-(2-benzothiazolyl)-2-naphthalenol fluorophore connected with a 4-aminophenol (the fluorescence quenching and response group). This probe exhibits a high selectivity and excellent sensitivity with a detection limit of 7.6nM over other reactive oxygen species and analyte species, and the fluorescence intensity enhanced 103-fold when responsed. Furthermore, it was successfully used for two-photon imaging of endogenous HClO in live cells with high-resolution.

Keywords: Hypochlorous acid; Reactive oxygen species (ROS); Two-photon fluorescent probe.