Viral Engineering of Chimeric Antigen Receptor Expression on Murine and Human T Lymphocytes

Joanne A Hammill; Arya Afsahi; Jonathan L Bramson; Christopher W Helsen

doi:10.1007/978-1-4939-3801-8_11

Viral Engineering of Chimeric Antigen Receptor Expression on Murine and Human T Lymphocytes

Methods Mol Biol. 2016:1458:137-57. doi: 10.1007/978-1-4939-3801-8_11.

Authors

Joanne A Hammill¹, Arya Afsahi¹, Jonathan L Bramson², Christopher W Helsen¹

Affiliations

¹ Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre, McMaster University, 1200 Main Street West, Hamilton, ON, Canada, L8N 3Z5.
² Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre, McMaster University, 1200 Main Street West, Hamilton, ON, Canada, L8N 3Z5. bramsonj@mcmaster.ca.

PMID: 27581020
DOI: 10.1007/978-1-4939-3801-8_11

Abstract

The adoptive transfer of a bolus of tumor-specific T lymphocytes into cancer patients is a promising therapeutic strategy. In one approach, tumor specificity is conferred upon T cells via engineering expression of exogenous receptors, such as chimeric antigen receptors (CARs). Here, we describe the generation and production of both murine and human CAR-engineered T lymphocytes using retroviruses.

Keywords: CAR-T cell; Chimeric antigen receptor; Gamma-retrovirus; Lentivirus; T lymphocytes.

MeSH terms

Animals
Cell Culture Techniques
Gene Transfer Techniques
Genetic Engineering*
Genetic Vectors / genetics*
Humans
Immunotherapy, Adoptive
Lentivirus / genetics
Mice
Neoplasms / immunology
Neoplasms / therapy
Receptors, Antigen, T-Cell / genetics*
Receptors, Antigen, T-Cell / metabolism
Recombinant Fusion Proteins / genetics*
Recombinant Fusion Proteins / metabolism
Retroviridae / genetics
T-Lymphocytes / immunology*
T-Lymphocytes / metabolism*
Transduction, Genetic

Substances

Receptors, Antigen, T-Cell
Recombinant Fusion Proteins