Objectives: To evaluate four 5'-UTRs on GFP expression in HEK293T cells.
Results: The recombinant plasmids were constructed by restriction enzyme digestion, digestion and DNA sequencing. Quantitative real-time PCR and western blotting results showed that the transcription and translation level of PPRV-GFP mRNA was significantly lower than that of the other reporters. The transcription and translation level of ChEF1-GFP was the highest in HEK293T cells.
Conclusions: Different UTRs can significantly affect protein expression. Additionally, the findings also will be useful in biological applications that require tuning of gene expression and system optimization.
Keywords: Gene expression; Green fluorescent protein (GFP) expression; Untranslated regions (UTRs); Vectors; Viruses.