Prevalence of Quinolone Resistance in Enterobacteriaceae from Sierra Leone and the Detection of qnrB Pseudogenes and Modified LexA Binding Sites

Tomasz A Leski; Michael G Stockelman; Umaru Bangura; Daniel Chae; Rashid Ansumana; David A Stenger; Gary J Vora; Chris R Taitt

doi:10.1128/AAC.01576-16

Prevalence of Quinolone Resistance in Enterobacteriaceae from Sierra Leone and the Detection of qnrB Pseudogenes and Modified LexA Binding Sites

Antimicrob Agents Chemother. 2016 Oct 21;60(11):6920-6923. doi: 10.1128/AAC.01576-16. Print 2016 Nov.

Authors

Tomasz A Leski¹, Michael G Stockelman², Umaru Bangura³, Daniel Chae⁴, Rashid Ansumana^{3

5}, David A Stenger², Gary J Vora², Chris R Taitt²

Affiliations

¹ Center for Bio/Medical Science and Engineering, Naval Research Laboratory, Washington, DC, USA tomasz.leski@nrl.navy.mil.
² Center for Bio/Medical Science and Engineering, Naval Research Laboratory, Washington, DC, USA.
³ Mercy Hospital Research Laboratory, Bo, Sierra Leone.
⁴ Thomas Jefferson High School for Science and Technology, Alexandria, Virginia, USA.
⁵ Institute of Environmental Management and Quality Control, Njala University, Bo, Sierra Leone.

Abstract

A collection of 74 Enterobacteriaceae isolates found in Bo, Sierra Leone, were tested for quinolone antibiotic susceptibility and resistance mechanisms. The majority of isolates (62%) were resistant to quinolones, and 61% harbored chromosomal gyrA and/or parC mutations. Plasmid-mediated quinolone resistance genes were ubiquitous, with qnrB and aac(6')-Ib-cr being the most prevalent. Mutated LexA binding sites were found in all qnrB1 genes, and truncated qnrB pseudogenes were found in the majority of Citrobacter isolates.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Binding Sites
DNA Gyrase / genetics
DNA Topoisomerase IV / genetics
Drug Resistance, Bacterial / drug effects
Drug Resistance, Bacterial / genetics*
Enterobacteriaceae / drug effects*
Enterobacteriaceae / genetics
Enterobacteriaceae / isolation & purification
Enterobacteriaceae Infections / epidemiology
Enterobacteriaceae Infections / microbiology
Humans
Microbial Sensitivity Tests
Mutation
Pseudogenes
Quinolones / pharmacology*
Serine Endopeptidases / metabolism*
Sierra Leone / epidemiology

Substances

Anti-Bacterial Agents
Bacterial Proteins
LexA protein, Bacteria
Quinolones
Serine Endopeptidases
DNA Topoisomerase IV
DNA Gyrase

Grants and funding

This work was supported in part by the Joint Science and Technology Office (JSTO), Defense Threat Reduction Agency (DTRA), and the Office of Naval Research (ONR) via U.S. Naval Research Laboratory core funds. The funding entities had no role in or any influence over the design of the study, collection, analysis, and interpretation of data, or the final manuscript. One of the funding agencies (DTRA) requested the inclusion of the following distribution statement to this manuscript: “Approved for public release; distribution is unlimited.” This reflects the unclassified nature of the presented data.