Technological approach of 1-O-alkyl-sn-glycerols separation from Berryteuthis magister squid liver oil

Ekaterina Ermolenko; Nikolay Latyshev; Ruslan Sultanov; Sergey Kasyanov

doi:10.1007/s13197-015-2148-x

Technological approach of 1-O-alkyl-sn-glycerols separation from Berryteuthis magister squid liver oil

J Food Sci Technol. 2016 Mar;53(3):1722-6. doi: 10.1007/s13197-015-2148-x. Epub 2015 Dec 22.

Authors

Ekaterina Ermolenko¹, Nikolay Latyshev¹, Ruslan Sultanov¹, Sergey Kasyanov²

Affiliations

¹ A.V. Zhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, 690041 17 Palchevskogo St., Vladivostok, Russia.
² A.V. Zhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, 690041 17 Palchevskogo St., Vladivostok, Russia ; Far Eastern Federal University (FEFU), 690950 8 Suhanova St., Vladivostok, Russia.

Abstract

Biological active compounds, 1-O-alkyl-sn-glycerols (AG), were isolated from liver oil of the squid Berryteuthis magister. The main components of the initial lipids were 1-O-alkyl-2,3-diacyl-sn-glycerols (38.50 %) and triacylglycerols (24.26 %). The first step of separation was the alkaline hydrolysis of oil to form a lipid mixture consisting of AG, free fatty acids and cholesterol. AG were separated by double recrystallization from acetone at -20 °C and 1 °C. A simple procedure is proposed for obtaining AG with a purity of 99.22 %, the main component of which is chimyl alcohol (94.39 %). Purity and structure of the obtained products were confirmed by GC and GC-MS technique. Isolated AG may be used in nutrition and cosmetics.

Keywords: 1-O-alkyl-sn-glycerols; Biologically active compounds; Low temperature crystallization; Marine lipids.