[Performance evaluation of Rapid™ Yeast Plus (Remel) system from two different culture media]

Abstract

Within the genus Candida, Candida albicans is the most commonly isolated species from clinical samples. Due to the emergence of other species which can show a higher index of antifungal resistance, a fast identification of these species is necessary. The aim of this work was to evaluate the performance of the RapID Yeast Plus system from two different subculture media formulations: Sabouraud dextrose agar adjusted by Emmons (the medium is indicated in the equipment insert) and Sabouraud glucose agar, which is the most frequently used in Buenos Aires City laboratories. One hundred and sixty-six clinical sample strains coming from different hospitals belonging to the Mycology Network of Buenos Aires City were studied. From the obtained results, we conclude that the conditions and culture medium indicated by the manufacturer should be followed.

Keywords: Candida spp.; Final yeast identification; Identificación definitiva de levaduras; Rapid™ Yeast Plus; Rapid™ Yeast Plus, Candida spp.; Sabouraud dextrosa modificado por Emmons; Sabouraud dextrose adjusted by Emmons.