IL-8 interacts with metadherin promoting proliferation and migration in gastric cancer

Biochem Biophys Res Commun. 2016 Sep 23;478(3):1330-7. doi: 10.1016/j.bbrc.2016.08.123. Epub 2016 Aug 24.

Abstract

It has been reported that IL-8 was involved in the promotion of invasion of Gastric Cancer (GC), however the underlying mechanism by which IL-8 was observed to be able to promote invasion remains unknown. Here, in our study, IL-8 was shown to be significantly up-regulated in GC compared with paired normal control tissues whose expression was markedly associated with inferior overall prognosis; and IL-8 was displayed to be capable of directly interacting with metadherin (MTDH), which in turn can up-regulate IL-8 expression. Blockage of IL-8/MTDH using specific mono-antibody can abolish the invasion IL-8 mediated. Taken together, our results may provide a novel explanation of working mechanism of IL-8 in the invasion of GC.

Keywords: Gastric cancer; IL-8; Invasion; MTDH; Prognosis.

MeSH terms

  • Case-Control Studies
  • Cell Adhesion Molecules / metabolism*
  • Cell Line, Tumor
  • Cell Movement*
  • Cell Proliferation
  • Female
  • Humans
  • Interleukin-8 / metabolism*
  • Male
  • Membrane Proteins
  • Middle Aged
  • Neoplasm Invasiveness
  • Neutralization Tests
  • Protein Binding
  • RNA-Binding Proteins
  • Stomach Neoplasms / metabolism*
  • Stomach Neoplasms / pathology*
  • Up-Regulation

Substances

  • Cell Adhesion Molecules
  • Interleukin-8
  • MTDH protein, human
  • Membrane Proteins
  • RNA-Binding Proteins