Impact of parity on the efficiency of ovulation synchronization protocols in Holstein cows

Abstract

The objective of this study was to elucidate the impact of parity on the efficiency of three different protocols to synchronize time of ovulation in Holstein cows. All cows enrolled in this trial were categorized into primiparous and multiparous (M₁ = 2-3 and M₂ ≥3 parities). Conception (P/AI at Day 28) and pregnancy (P/AI at Day 75) rates in the Presynch and CIDRsynch (31.7% and 35.5%; 26.3% and 28.4%, respectively) groups were significantly greater than that in spontaneous estrus (SE; 24.7 and 20.4%, respectively) group (P = 0.048 and 0.024; 0.041 and 0.011, respectively); however, no significant differences were detected between the SE and Ovsynch group for conception, pregnancy, and embryonic loss rates. Conception and pregnancy rates in the Presynch group decreased from 37.8% and 33.5%, respectively in primiparous cows to 29.6% and 23.1%, respectively in M₁ cows (P = 0.022 and 0.007, respectively). However, conception and pregnancy rates using the CIDRsynch regimen were increased from 31.1% and 23.8% in primiparous cows to 41.4% and 34.7% in M₁ cows (P = 0.017 and 0.008, respectively), and 40.9% and 33.6% in M₂ cows (P = 0.021 and 0.019, respectively). Embryonic loss rate using the CIDRsynch protocol was decreased from 22.9% in primiparous cows to 15.1% and 18.2% in M₁ and M₂ cows (P = 0.013 and 0.130, respectively). On the contrary, embryonic loss rate using the Ovsynch protocol was increased from 11.5% in primiparous cows to 22.1% and 21.8% in M₁ and M₂ cows (P = 0.001 and 0.003, respectively). The Cox proportional-hazards model of embryonic loss showed significant associations for parity and season of calving with the hazard of embryonic loss (P = 0.001 and 0.016, respectively). Multiparous cows (M1 and M2) had a higher risk of embryonic loss than primiparous cows (Hazard ratio = 1.32 and 1.89, respectively). Our results indicate that use of the CIDRsynch regimen may achieve satisfactory conception and pregnancy rates in multiparous Holstein cows. However, synchronizing time of ovulation in primiparous cows with use of the Presynch treatment increases the fertility indices.

Keywords: Estrous synchronization; Fertility; Holstein; Parity.