The thioredoxin MoTrx2 protein mediates reactive oxygen species (ROS) balance and controls pathogenicity as a target of the transcription factor MoAP1 in Magnaporthe oryzae

Abstract

We have shown previously that the transcription factor MoAP1 governs the oxidative response and is important for pathogenicity in the rice blast fungus Magnaporthe oryzae. To explore the underlying mechanism, we have identified thioredoxin MoTrx2 as a target of MoAP1 in M. oryzae. Thioredoxins are highly conserved 12-kDa oxidoreductase enzymes containing a dithiol-disulfide active site, and function as antioxidants against free radicals, such as reactive oxygen species (ROS). In yeast and fungi, thioredoxins are important for oxidative stress tolerance and growth. To study the functions of MoTrx2, we generated ΔMotrx2 mutants that exhibit various defects, including sulfite assimilation, asexual and sexual differentiation, infectious hyphal growth and pathogenicity. We found that ΔMotrx2 mutants possess a defect in the scavenging of ROS during host cell invasion and in the active suppression of the rice defence response. We also found that ΔMotrx2 mutants display higher intracellular ROS levels during conidial germination, but lower peroxidase and laccase activities, which contribute to the attenuation in virulence. Given that the function of MoTrx2 overlaps that of MoAP1 in the stress response and pathogenicity, our findings further indicate that MoTrx2 is a key thioredoxin protein whose function is subjected to transcriptional regulation by MoAP1 in M. oryzae.

Keywords: Magnaporthe oryzae; MoAP1; ROS balance; pathogenicity; sulfite assimilation; thioredoxin.