Complete genome sequence of thermophilic Bacillus smithii type strain DSM 4216(T)

Elleke F Bosma; Jasper J Koehorst; Sacha A F T van Hijum; Bernadet Renckens; Bastienne Vriesendorp; Antonius H P van de Weijer; Peter J Schaap; Willem M de Vos; John van der Oost; Richard van Kranenburg

doi:10.1186/s40793-016-0172-8

Complete genome sequence of thermophilic Bacillus smithii type strain DSM 4216(T)

Stand Genomic Sci. 2016 Aug 24;11(1):52. doi: 10.1186/s40793-016-0172-8. eCollection 2016.

Affiliations

¹ Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands ; Present address: The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Kgs. Lyngby, 2800 Denmark.
² Laboratory of Systems and Synthetic Biology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
³ CMBI, NCMLS, Geert-Grooteplein Zuid 26-28, 6525 GA Nijmegen, The Netherlands.
⁴ Corbion, Arkelsedijk 46, 4206 AC Gorinchem, The Netherlands.
⁵ Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
⁶ Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands ; Corbion, Arkelsedijk 46, 4206 AC Gorinchem, The Netherlands.

Abstract

Bacillus smithii is a facultatively anaerobic, thermophilic bacterium able to use a variety of sugars that can be derived from lignocellulosic feedstocks. Being genetically accessible, it is a potential new host for biotechnological production of green chemicals from renewable resources. We determined the complete genomic sequence of the B. smithii type strain DSM 4216(T), which consists of a 3,368,778 bp chromosome (GenBank accession number CP012024.1) and a 12,514 bp plasmid (GenBank accession number CP012025.1), together encoding 3880 genes. Genome annotation via RAST was complemented by a protein domain analysis. Some unique features of B. smithii central metabolism in comparison to related organisms included the lack of a standard acetate production pathway with no apparent pyruvate formate lyase, phosphotransacetylase, and acetate kinase genes, while acetate was the second fermentation product.

Keywords: Bacillus smithii; Biotechnology; Genome sequence; Lactic acid; Thermophile; Thermophilic bacillus.

Publication types

Case Reports