Establishment of induced pluripotent stem cell (iPSC) line from an 84-year old patient with late onset Alzheimer's disease (LOAD)

Zsuzsanna Táncos; Eszter Varga; Eszter Kovács; András Dinnyés; Julianna Kobolák

doi:10.1016/j.scr.2016.05.016

Establishment of induced pluripotent stem cell (iPSC) line from an 84-year old patient with late onset Alzheimer's disease (LOAD)

Stem Cell Res. 2016 Jul;17(1):75-77. doi: 10.1016/j.scr.2016.05.016. Epub 2016 May 24.

Authors

Zsuzsanna Táncos¹, Eszter Varga², Eszter Kovács², András Dinnyés¹, Julianna Kobolák³

Affiliations

¹ BioTalentum Ltd., Gödöllő, Hungary; Molecular Animal Biotechnology Laboratory, Szent István University, Gödöllő, Hungary.
² BioTalentum Ltd., Gödöllő, Hungary.
³ BioTalentum Ltd., Gödöllő, Hungary. Electronic address: julianna.kobolak@biotalentum.hu.

PMID: 27558606
DOI: 10.1016/j.scr.2016.05.016

Abstract

Peripheral blood mononuclear cells (PBMCs) were collected from a clinically characterised 84-year old male with late onset Alzheimer's disease (LOAD). The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system. The transgene-free iPSC showed pluripotency verified by immunocytochemistry for pluripotency markers and differentiated spontaneously towards the 3 germ layers in vitro. Furthermore, the iPSC line showed normal karyotype. Our model might offer a good platform to further study the pathomechanism of sporadic AD, to identify early biomarkers and also for drug testing and gene therapy studies.