Establishment of induced pluripotent stem cell (iPSC) line from a 57-year old patient with sporadic Alzheimer's disease

Anna Ochalek; Csilla Nemes; Eszter Varga; Zsuzsanna Táncos; Julianna Kobolák; András Dinnyés

doi:10.1016/j.scr.2016.05.020

Establishment of induced pluripotent stem cell (iPSC) line from a 57-year old patient with sporadic Alzheimer's disease

Stem Cell Res. 2016 Jul;17(1):72-74. doi: 10.1016/j.scr.2016.05.020. Epub 2016 May 25.

Authors

Anna Ochalek¹, Csilla Nemes², Eszter Varga², Zsuzsanna Táncos³, Julianna Kobolák², András Dinnyés³

Affiliations

¹ Molecular Animal Biotechnology Laboratory, Szent István University, Gödöllő, Hungary.
² BioTalentum Ltd., Gödöllő, Hungary.
³ Molecular Animal Biotechnology Laboratory, Szent István University, Gödöllő, Hungary; BioTalentum Ltd., Gödöllő, Hungary.

PMID: 27558605
DOI: 10.1016/j.scr.2016.05.020

Abstract

Peripheral blood mononuclear cells (PBMCs) were collected from a clinically characterised 57-year old woman with sporadic Alzheimer's disease. The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system. The transgene-free iPSC showed pluripotency verified by immunocytochemistry for pluripotency markers and differentiated spontaneously towards the 3 germ layers in vitro. Furthermore, the iPSC line showed normal karyotype. Our model might offer a good platform to further study the pathomechanism of sporadic AD, to identify early biomarkers and also for drug testing and gene therapy studies.