Derivation of the clinical grade human embryonic stem cell line RCe021-A (RC-17)

P A De Sousa; B J Tye; K Bruce; P Dand; G Russell; D M Collins; A Greenshields; K McDonald; H Bradburn; D Allan; A Laurie; T Kunath; M A Canham; J M Downie; M Bateman; A Courtney

doi:10.1016/j.scr.2016.04.019

Derivation of the clinical grade human embryonic stem cell line RCe021-A (RC-17)

Stem Cell Res. 2016 Jul;17(1):1-5. doi: 10.1016/j.scr.2016.04.019. Epub 2016 Apr 30.

Authors

P A De Sousa¹, B J Tye², K Bruce², P Dand², G Russell², D M Collins², A Greenshields², K McDonald², H Bradburn², D Allan², A Laurie², T Kunath³, M A Canham³, J M Downie², M Bateman², A Courtney²

Affiliations

¹ Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK; Centre for Clinical Brain Sciences, University of Edinburgh, UK; MRC Centre for Regenerative Medicine, University of Edinburgh, UK. Electronic address: paul.desousa@ed.ac.uk.
² Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
³ MRC Centre for Regenerative Medicine, University of Edinburgh, UK.

PMID: 27558596
DOI: 10.1016/j.scr.2016.04.019

Abstract

The human embryonic stem cell line RCe021-A (RC-17) was derived under quality assured compliance with UK regulation, European Union Directives and International guidance for tissue procurement, processing and storage according to Good Manufacturing Practice (GMP) standards. The cell line was derived from a day 3 embryo voluntarily donated as unsuitable or surplus to fertility requirements following informed consent. RCe021-A (RC-17) shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a normal 46XX female karyotype and microsatellite PCR identity, HLA and blood group typing data are available.

Grants and funding

MR/K017276/1/MRC_/Medical Research Council/United Kingdom