Gametocidal (Gc) system is a novel method of inducing chromosome mutations in wheat by using Gc genes of wheat-related species. Many deletion lines were efficiently produced by this system and used for physical mapping of genes and DNA markers. A large stocks with chromosome deletions in homozygous state as well as the lines with Gc genes are available in the gene bank of Japan (KOMUGI). In this chapter, I describe the method of inducing breakage in a target chromosome using lines with the Gc gene on chromosome 2C of Aegilops cylindrica (a wheat-related wild species) and nullisomic-tetrasomics.
Keywords: Addition line; Aegilops cylindrica; Chromosome dissection; Deletion stock; Gametocidal chromosome; KOMUGI; Nullisomic–tetrasomics.