Insights into molecular and cellular mechanisms of hormonal actions on fish ion regulation derived from the zebrafish model

Abstract

Fish have sophisticated mechanisms of ionic and acid-base regulation for maintaining body fluid homeostasis. Many hormones have been proposed to control the ionic and acid-base regulation mechanisms in fishes; however, lots of the proposed actions lack convincing cellular/molecular evidence. With the advantages of available genetic databases and molecular manipulation techniques, zebrafish has become an emerging model for research into ion transport physiology and functional regulation. Different types of ionocytes were found to transport ions through various sets of ion transporters, and the molecular mechanisms of ionocyte proliferation and differentiation have also been dissected, providing a competent platform with which to precisely study the ion transport pathways and ionocytes targeted by hormones, including isotocin, prolactin, cortisol, stanniocalcin-1, calcitonin, endothelin-1, vitamin D, parathyroid hormone 1, catecholamines, the renin-angiotensin-system, estrogen-related receptor α, and calcitonin gene-related peptide, which have been demonstrated to positively or negatively regulate ion transport through specific receptors at different molecular levels (transcriptional, translational, or posttranslational) or at different developmental stages of ionocytes (proliferation or differentiation). The knowledge obtained in zebrafish not only enhances our understanding of the hormonal control of fish ion regulation, but also informs studies on other animal species, thereby providing insights into related fields.

Keywords: Hormone; Ion regulation; Ionocyte; Zebrafish.