Abstract
Sialic acids are typically linked α(2-3) or α(2-6) to the galactose that located at the non-reducing terminal end of glycans, playing important but distinct roles in a variety of biological and pathological processes. However, details about their respective roles are still largely unknown due to the lack of an effective analytical technique. Herein, a two-step chemoenzymatic approach for the rapid and sensitive detection of N-acetylneuraminic acid-α(2-3)-galactose glycans is described.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Biosensing Techniques / methods*
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Campylobacter jejuni / enzymology
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Galactose / chemistry*
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HEK293 Cells
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Humans
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Limit of Detection
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N-Acetylgalactosaminyltransferases / metabolism*
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N-Acetylneuraminic Acid / chemistry*
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Polysaccharides / chemistry*
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Polysaccharides / metabolism*
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Substrate Specificity
Substances
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Polysaccharides
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N-Acetylgalactosaminyltransferases
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N-Acetylneuraminic Acid
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Galactose