Is there a role for stool metabolomics in cystic fibrosis?

Nadeem O Kaakoush; Russell Pickford; Adam Jaffe; Chee Y Ooi

doi:10.1111/ped.13063

Is there a role for stool metabolomics in cystic fibrosis?

Pediatr Int. 2016 Aug;58(8):808-11. doi: 10.1111/ped.13063.

Authors

Nadeem O Kaakoush¹, Russell Pickford², Adam Jaffe^{3

4}, Chee Y Ooi^{3

5}

Affiliations

¹ School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia.
² Bioanalytical Mass Spectrometry Facility, University of New South Wales, Sydney, New South Wales, Australia.
³ Discipline of Paediatrics, School of Women's and Children's Health, Medicine, University of New South Wales, Sydney, New South Wales, Australia.
⁴ Department of Paediatric Respiratory, Sydney Children's Hospital Randwick, Randwick, New South Wales, Australia.
⁵ Department of Paediatric Gastroenterology, Sydney Children's Hospital Randwick, Randwick, New South Wales, Australia.

PMID: 27553892
DOI: 10.1111/ped.13063

Abstract

A number of studies utilizing metabolomics have focused on the pathophysiology of cystic fibrosis (CF) lung disease. Here, we performed fecal metabolomics on pancreatic insufficient (PI) and sufficient (PS) children with CF and compared them with healthy controls (HC). Fecal metabolomics can differentiate between PS-CF and PI-CF. We identified a potential biomarker of disease severity or cystic fibrosis transmembrane conductance regulator function (m/z, 463.247; retention time, 0.570717 min) that discriminates between HC versus PS-CF versus PI-CF. We also identified lipoyl-GMP as a potential novel inflammatory biomarker, and elevation in fecal glycerol 1,2-didodecanoate 3-tetradecanoate may provide clues to the pathogenesis of intestinal inflammation. For the first time, we demonstrate the potential applications of fecal metabolomics in CF.

Keywords: children; cystic fibrosis; exocrine pancreatic insufficiency; inflammation; metabolomics.

Publication types

Review

MeSH terms

Cystic Fibrosis / microbiology*
Feces / microbiology
Humans
Metabolomics / methods*