Histone deacetylase inhibitors induce invasion of human melanoma cells in vitro via differential regulation of N-cadherin expression and RhoA activity

BMC Cancer. 2016 Aug 22;16(1):667. doi: 10.1186/s12885-016-2693-3.

Abstract

Background: Histone deacetylase inhibitors (HDACi) exert multiple cytotoxic actions on cancer cells. Currently, different synthetic HDACi are in clinical use or clinical trials; nevertheless, since both pro-invasive and anti-invasive activities have been described, there is some controversy about the effect of HDACi on melanoma cells.

Methods: Matrigel and Collagen invasion assays were performed to evaluate the effect of several HDACi (Butyrate, Trichostatin A, Valproic acid and Vorinostat) on two human melanoma cell line invasion (A375 and HT-144). The expression of N- and E-Cadherin and the activity of the RhoA GTPase were analyzed to elucidate the mechanisms involved in the HDACi activity.

Results: HDACi showed a pro-invasive effect on melanoma cells in vitro. This effect was accompanied by an up-regulation of N-cadherin expression and an inhibition of RhoA activity. Moreover, the down-regulation of N-cadherin through blocking antibodies or siRNA abrogated the pro-invasive effect of the HDACi and, additionally, the inhibition of the Rho/ROCK pathway led to an increase of melanoma cell invasion similar to that observed with the HDACi treatments.

Conclusion: These results suggest a role of N-cadherin and RhoA in HDACi induced invasion and call into question the suitability of some HDACi as antitumor agents for melanoma patients.

Keywords: Cell invasion; HDACi; Histone deacetylase inhibitors; Melanoma; N-cadherin; RhoA.

MeSH terms

  • Antineoplastic Agents / pharmacology
  • Apoptosis / drug effects
  • Butyrates / pharmacology
  • Cadherins / biosynthesis*
  • Cadherins / genetics
  • Cell Line, Tumor
  • Gene Expression Regulation, Neoplastic
  • Histone Deacetylase Inhibitors / pharmacology*
  • Humans
  • Hydroxamic Acids / pharmacology
  • Melanoma / pathology*
  • Neoplasm Invasiveness / pathology*
  • RNA Interference
  • RNA, Small Interfering / genetics
  • Valproic Acid / pharmacology
  • Vorinostat
  • rhoA GTP-Binding Protein / metabolism*

Substances

  • Antineoplastic Agents
  • Butyrates
  • Cadherins
  • Histone Deacetylase Inhibitors
  • Hydroxamic Acids
  • RNA, Small Interfering
  • RHOA protein, human
  • trichostatin A
  • Vorinostat
  • Valproic Acid
  • rhoA GTP-Binding Protein