In vitro BMP-2 peptide release from thiolated chitosan based hydrogel

Xujie Liu; Bo Yu; Qianli Huang; Rui Liu; Qingling Feng; Qiang Cai; Shengli Mi

doi:10.1016/j.ijbiomac.2016.08.048

In vitro BMP-2 peptide release from thiolated chitosan based hydrogel

Int J Biol Macromol. 2016 Dec;93(Pt A):314-321. doi: 10.1016/j.ijbiomac.2016.08.048. Epub 2016 Aug 17.

Authors

Xujie Liu¹, Bo Yu², Qianli Huang³, Rui Liu², Qingling Feng⁴, Qiang Cai⁵, Shengli Mi⁶

Affiliations

¹ Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China; State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.
² Department of Orthopedics, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, China.
³ State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.
⁴ State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China; Key Laboratory of Advanced Materials of Ministry of Education of China, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China. Electronic address: biomater@mail.tsinghua.edu.cn.
⁵ Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China; State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China; Key Laboratory of Advanced Materials of Ministry of Education of China, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.
⁶ Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China. Electronic address: mi.shengli@sz.tsinghua.edu.cn.

PMID: 27544436
DOI: 10.1016/j.ijbiomac.2016.08.048

Abstract

Thiolated chitosan based thermo-sensitive hydrogel is a water soluble system and the existing thiol groups are beneficial for the delivery of cysteine-rich peptides. In the present study, a kind of thiolated chitosan, i.e. chitosan-4-thio-butylamidine (CS-TBA) conjugate was characterized and used to prepare CS-TBA/hydroxyapatite (HA)/beta-glycerophosphate disodium (β-GP) thermo-sensitive hydrogel. The cysteine terminated peptide 24 (P24) containing residues 73-92 of the knuckle epitope of BMP-2 (N→C: KIPKASSVPTELSAISTLYLSGGC) was synthesized and characterized. The release behavior of P24 from CS-TBA based hydrogel was investigated in vitro. The thiol groups in CS-TBA may react with thiol groups in P24, thus decreases the P24 release rate and maintains the peptide release for a longer time compared with unmodified chitosan based hydrogel. Moreover, the bioactivity of P24 is preserved during release process. These results indicate that P24 loaded CS-TBA based thermosensitive hydrogel is a potential material for minimally invasive surgery of bone repair.

Keywords: BMP-2 peptide release; Bone tissue engineering; Thiolated chitosan.

MeSH terms

Amino Acid Sequence
Animals
Bone Morphogenetic Protein 2 / chemistry*
Chitin / analogs & derivatives*
Chitin / chemistry
Drug Carriers / chemistry*
Drug Liberation*
Female
Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry*
Peptide Fragments / chemistry*
Peptide Fragments / pharmacology
Rats

Substances

Bone Morphogenetic Protein 2
Drug Carriers
Peptide Fragments
chitosan-4-thiobutylamidine
Chitin
Hydrogel, Polyethylene Glycol Dimethacrylate