The long-term survival of relict populations depends on the accessibility of appropriate sites (microrefugia). In recent times, due to the mass extinction of rare species that has resulted from the loss of natural habitats, the question is - Are there any human-made sites that can act as refugial habitats? We examined forest roadside populations of the mountain plant Pulsatilla vernalis in the last large lowland refugium in Central Europe. We compared the habitat conditions and community structure of roadsides with P. vernalis against the forest interior. Light availability and bryophyte composition were the main factors that distinguished roadsides. Pulsatilla occurred on sites that had more light than the forest interior, but were also more or less shaded by trees, so more light came as one-side illumination from the road. Roadsides had also a lower coverage of bryophytes that formed large, dense carpets. At the same time, they were characterised by a greater richness of vascular plants and 'small' bryophytes, which corresponds to a higher frequency of disturbances. In a warming and more fertile Anthropocene world, competition plays the main role in the transformation of forest communities, which is why relict populations have found refugia in extensively disturbed human-made habitats.