Interleukin-18 Binding Protein Pretreatment Attenuates Kidney Injury Induced by Hepatic Ischemia Reperfusion

Yucel Gonul; Senem Kazandı; Ahmet Kocak; Ahmet Ahsen; Ahmet Bal; Afra Karavelioglu; Omer Hazman; Ozan Turamanlar; Serdar Kokulu; Seref Yuksel

doi:10.1016/j.amjms.2016.04.012

Interleukin-18 Binding Protein Pretreatment Attenuates Kidney Injury Induced by Hepatic Ischemia Reperfusion

Am J Med Sci. 2016 Aug;352(2):200-7. doi: 10.1016/j.amjms.2016.04.012. Epub 2016 Apr 21.

Authors

Yucel Gonul¹, Senem Kazandı², Ahmet Kocak³, Ahmet Ahsen⁴, Ahmet Bal⁵, Afra Karavelioglu⁶, Omer Hazman⁷, Ozan Turamanlar², Serdar Kokulu⁸, Seref Yuksel⁴

Affiliations

¹ Department of Anatomy, Afyon Kocatepe University, Afyon, Turkey. Electronic address: yucel94@hotmail.com.
² Department of Anatomy, Afyon Kocatepe University, Afyon, Turkey.
³ Department of Histology and Embryology, Dumlupınar University, Kütahya, Turkey.
⁴ Department of Nephrology, Afyon Kocatepe University, Afyon, Turkey.
⁵ Department of General Surgery, Afyon Kocatepe University, Afyon, Turkey.
⁶ Department of Pediatric Surgery, Afyon Kocatepe University, Afyon, Turkey.
⁷ Department of Biochemistry, Afyon Kocatepe University, Afyon, Turkey.
⁸ Department of Anesthesia and Reanimation, Afyon Kocatepe University, Afyon, Turkey.

PMID: 27524219
DOI: 10.1016/j.amjms.2016.04.012

Abstract

Objective: Acute kidney injury (AKI) is a serious condition that can be induced by liver transplantation, major hepatic resection or prolonged portal vein occlusion. AKI can increase the frequency of postoperative complications. In the current study, we aimed to investigate whether interleukin-18 binding protein (IL-18BP) pretreatment has a protective effect against possible kidney injury following liver ischemia-reperfusion (IR) achieved by Pringle maneuver in an experimental rat model.

Materials and methods: A total of 24 male Wistar albino rats were included in this study. Animals were equally and randomly separated into 3 groups as follows: I, Sham group, II, IR group (1-hour ischemia and 4-hour reperfusion) and III, IR + IL-18BP group (50μg/kg IL-18BP was intraperitoneally administered 30 minutes before surgery). Blood, liver and kidney samples were collected for histopathological and biochemical (hepatic and renal function, nitric oxide, malondialdehyde and glutathione levels) analysis. In addition, proinflammatory cytokines including tumor necrosis factor α, IL-1β and IL-6 levels were measured in kidney tissues.

Results: IL-18BP has improved kidney functions in acute kidney damage, restored structural changes, exhibited anti-inflammatory effects by decreasing proinflammatory cytokines and regulated the oxidative stress parameters by antioxidant effect.

Conclusions: Current study would be the first to evaluate the protective, antioxidant and anti-inflammatory effects of IL-18BP on renal damage induced by liver ischemia (1 hour) and reperfusion (4 hours). As a result, we have demonstrated that AKI may develop after hepatic IR with Pringle maneuver and IL-18BP pretreatment can attenuate this damage. By this way, complications related to liver IR could be minimized and also postoperative hospitalization durations, treatment costs and healing periods could be decreased.

Keywords: Acute kidney injury; Inflammation; Interleukin-18 binding protein; Liver ischemia-reperfusion; Oxidative stress.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acute Kidney Injury / etiology
Acute Kidney Injury / pathology
Acute Kidney Injury / prevention & control*
Animals
Humans
Intercellular Signaling Peptides and Proteins / administration & dosage*
Liver Diseases / complications
Liver Diseases / drug therapy*
Liver Diseases / pathology
Male
Rats
Rats, Wistar
Reperfusion Injury / complications
Reperfusion Injury / drug therapy*
Reperfusion Injury / pathology

Substances

Intercellular Signaling Peptides and Proteins
interleukin-18 binding protein