Structure and bioactivity of a polysaccharide containing uronic acid from Polyporus umbellatus sclerotia

Pengfei He; Anqiang Zhang; Fuming Zhang; Robert J Linhardt; Peilong Sun

doi:10.1016/j.carbpol.2016.07.010

Structure and bioactivity of a polysaccharide containing uronic acid from Polyporus umbellatus sclerotia

Carbohydr Polym. 2016 Nov 5:152:222-230. doi: 10.1016/j.carbpol.2016.07.010. Epub 2016 Jul 4.

Authors

Pengfei He¹, Anqiang Zhang², Fuming Zhang³, Robert J Linhardt⁴, Peilong Sun⁵

Affiliations

¹ College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China; College of Ocean, Zhejiang University of Technology, Hangzhou 310032, China.
² College of Ocean, Zhejiang University of Technology, Hangzhou 310032, China; Departments of Chemistry and Chemical Biology and Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA. Electronic address: zhanganqiang@zjut.edu.cn.
³ Departments of Chemistry and Chemical Biology and Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
⁴ Departments of Chemistry and Chemical Biology and Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA; Department of Biological Science, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
⁵ College of Ocean, Zhejiang University of Technology, Hangzhou 310032, China. Electronic address: sun_pl@zjut.edu.cn.

PMID: 27516268
DOI: 10.1016/j.carbpol.2016.07.010

Abstract

Polyporus umbellatus is a medicinal fungus, has been used in traditional Chinese medicine for thousands years for treatment of edema, scanty urine, vaginal discharge, jaundice and diarrhea. The structure of a soluble polysaccharide (named PUP80S1), purified from the sclerotia of Polyporus umbellatus was elucidated by gas chromatography (GC), GC-mass spectrometry and nuclear magnetic resonance spectroscopy. PUP80S1 is a branched polysaccharide containing approximately 8.5% uronic acid and having an average molecular weight of 8.8kDa. Atomic force microscopy of PUP80S1 reveals a globular chain conformation in water. Antioxidant tests, Oxygen radical absorption capacity and 2,2-diphenyl-1-picrylhydrazyl radical scavenging assays indicate that PUP80S1 possesses significant antioxidant activity. But the related polysaccharide, PUP60S2, which contains more uronic acid residues and a higher level of branching, shows better antioxidant activity. These results suggest that structure features of polysaccharides play an important role in their physiological functions.

Keywords: Antioxidant activity; Atomic force microscopy; Nuclear magnetic resonance spectroscopy; Polyporus umbellatus; Polysaccharide; Structure-function relationship.

MeSH terms

Antioxidants / chemical synthesis
Antioxidants / chemistry
Antioxidants / pharmacology
Biphenyl Compounds / chemistry
Dietary Carbohydrates / chemical synthesis
Dietary Carbohydrates / pharmacology
Gas Chromatography-Mass Spectrometry
Magnetic Resonance Spectroscopy
Microscopy, Atomic Force
Molecular Structure
Oxygen Radical Absorbance Capacity
Picrates / chemistry
Polyporus / chemistry*
Polysaccharides / chemistry*
Polysaccharides / pharmacology*
Uronic Acids / chemistry*

Substances

Antioxidants
Biphenyl Compounds
Dietary Carbohydrates
Picrates
Polysaccharides
Uronic Acids
1,1-diphenyl-2-picrylhydrazyl